Materials

Organelle FAP Tags
pcDNA3.1-COX4-COX8-dL5-2XG4S-mCer3 (Mitochondria Addgene 73208)
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer-KDEL (Endoplasmic Reticulum Addgene 73209)
pcDNA3.1-KozATG-dL5-2XG4S-mCer3 (Cytosolic Addgene 73207)
pcDNA3.1-myc-dL5-2XG4S-mCerSKL (Peroxisomal Addgene 73210)
pcDNA3.1-NLS-myc-dL5-2XG4S-mCer3 (Nuclear Addgene 73205)

Actin FAP Tags
pcDNA3.1-KozATG-dH6.2-2XG4S-actin Addgene 73263
pcDNA3.1-KozATG-dL5-2XG4S-actin Addgene 73262

Membrane Protein FAP Tags
pcDNA3.1-kappa-dL5-2XG4S-TMst (PDGFR TM anchored surface-expression FAP Addgene 73206)
pcDNA3.1-kappa-HA-dL5-BKa (KCNMA1 Addgene 73212)
pBabe-kappa-HA-dL5-myc-ADRB2 (B2AR Addgene 101253)

FAP dL5 bacterial expression (Generally, we express from oxidizing strains such as Rosetta B-Gami2)
pET21a-10XHis-GST-HRV-dL5(YW) (Addgene 74214)

Affibodies and Affi-FAP constructs (See Wang, Y et al, Bioconj. Chem. 2015)
pET21a-10XHis-GST-HRV-dHer1cysteineST (double Her1-affibody Z1907 with cysteine at C-terminus Addgene 73218)
pET21a-10XHis-GST-HRV-dL5-Her1 (FAP-Affibody construct, Her1 Z1907 affibeody Addgene 73215)
pET21a-10XHis-GST-HRV-Her1-dL5-Her1 (Affibody-FAP-Affibody construct, Her1 affibeody Z1907 Addgene 73217)
pET21a-10XHis-GST-HRV-Her1-dL5 (Affibody-FAP construct, Her1 affibody Z1907 Addgene 73216)

MG-Clones-Spectra
TCM-HCM-Spectra