This microscope system provides one-photon (1-p) confocal, photo-manipulation and spectral unmixing fluorescence technologies. There are six laser lines (405, 458, 488, 514, 561 and 633 nm). Image acquisition includes multi-color, z-stack, time lapse and multi-position with hardware autofocusing, which is suitable for applications such as localization, colocalization, time-lapse, uncaging, FRET, FRAP, and ratio imaging. The instrument also has fluorescence correlation spectroscopy capabilities. Schedule.
This microscope system provides one-photon (1-p) and two-photon (2-p) confocal, fluorescence correlation spectroscopy (FCS), and spectra unmixing fluorescence technologies. There are five visible laser lines (458, 488, 514, 561, 633 nm) and a Chameleon Ultra 2-p laser (690-1020 nm) for excitation. Applications are broad with unique features such as 1-p/2-p FCS and confocal. Schedule.
This system provides a range of laser-illuminated microscopy solutions, including spinning disk confocal, total internal reflection fluorescence (TIRF) and FRAPPA (photo-bleach, activation and ablation) modalities, ideal for live-cell imaging and photo-manipulation. There are four laser lines (405, 488, 560, and 640 nm) and three cameras (two EMCCD and one CCD). Schedule.
The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing starting at 10 ng DNA, and HLA typing. New MiSeq reagents enable up to 15 Gb of output with 25 M sequencing reads and 2×300 bp read lengths. Schedule.
The BD FACSDiva workstation controls the BD FACSVantage SE flow cytometer when operated in digital mode. It consists of a Windows-based computer running BD FACSDiva acquisition and analysis software. Available laser excitations include 405, 488, 532 and 633 nm. The instrument can sort at the level of 100,000 events per second. Schedule.
The Intellicyt front end allows rapid sampling of 96-well plates and feed of these samples to the attached Accuri C6 flow analyzer to produce high-throughput single cell analysis data of up to two fluorescence parameters. The Accuri is equipped with two excitation lasers, 488 and 640 nm. Schedule.
Safire2 is a fully modular monochromator-based microplate detection system that offers a range of high-speed fluorescence techniques. Detection modules are available for top and bottom fluorescence intensity measurements, fluorescence polarization studies and multi-channel absorbance and luminescence measurements. Whether researchers wish to perform the latest biomolecular assays for primary or secondary screening, such as molecular interaction assays, kinase assays, protease assays or highly sensitive G-protein-coupled receptor assays, Safire2 has the options to fit the application requirements. Consecutive measurements in different fluorescent modes may be made with the shortest possible time interval between measurements. Safire2 also saves time and costs by eliminating the need for cumbersome filter changes. All wavelengths are easily accessible through a specially designed fast-scanning monochromator. Schedule.
Highly sensitive, second generation version of the Safire 2, equipped with fluorescence, luminescence, polarization and dual injection ports for kinetic and spectral analysis. Schedule.
Imaris scientific software module allows visualization of original and derived data objects in a real time interactive manner so you can quickly make visual assessments of your experiments in 3D and 4D to discover relationships that are otherwise hidden. Its rendering quality, speed, precision and interactivity are unrivalled. With a large variety of segmentation options, Imaris provides you with the most effective tools to segment even the toughest datasets to identify, separate, and visualize individual objects and then utilize the Imaris MeasurementPro module to pull out the statistics. A dedicated workstation with the software installed is available in the shared Imaging Facility. Software can be installed on your own machine and accessed through a license server from any computer on a wired connection in Mellon Institute. Schedule.